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Due to the low biological value of proteins of common maize, it was reinitiated breeding for high protein quality maize (HQPM) using three genetic systems, namely: opaque-2 gene, endosperm modifier genes and enhancer genes, which are increasing lysine and tryptophan content in opaque-2 background In order to alleviate effect of abiotic and biotic stress factors, the genotypes with tolerance to those factors were included. Genetic resources originating from North, Central and South America, then West, Central and Southern Africa and gene bank of Maize Research Institute “Zemun Polje” were used. Combining breeding approaches in selection of genetic resources, field plot techniques and laboratory analysis, it was created large number of early QPM varieties, inbred lines and hybrids with modified endosperm and high yield potential under poor and good growing conditions. Created lines exhibited high combining ability in conventional and non-conventional hybrids. Yield trials showed that QPM hybrids are competing with commercial hybrids of common maize.

Maize is the principal crop and major staple food in the most African and South American countries. The main problem in human nutrition in developing countries, and in livestock feed in developed countries, is insufficient production and poor quality of cereal proteins. In the case of maize, due to the very low content of essential amino acids lysine and tryptophan in grain endosperm, biological value is very low, which is main limiting factor of common maize in human nutrition and feeding of monogastric animals. Quality protein maize (QPM) can help in solving of this problem. Maize production also faces serious constraints caused by agro-ecological conditions and poor socio-economic situation. To alleviate the effect of the constraints, selected genotypes with more desirable traits and appropriate field-plot techniques to create multiple-stress conditions, were used. It was found that, in downy mildew nursery distance up to 35 m from spreader plot is providing sufficient down load of spores for plant infection, provided that the testing breeding materials are planted towards to down-stream direction of the dominant wind. Using these breeding approaches large number of early, white and flint synthetics, composites and inbred lines were created with resistance or tolerance to downy mildew (DMR), maize streak virus (SR) and drought (DT). Created genotypes exhibited very good kernel modification and yield potential under low and normal inputs. In the case of synthetics and composites, besides tolerance to multiple stress factors, they were competing in yield with local QPM and normal maize checks. In the case of created inbred lines high combining ability was exhibited both in non-conventional and conventional maize hybrids. Trial data revealed that in the most cases the best entries were over-yielding the best checks.

The incrementation of lysine, tryptophan and maize protein nutritional value in locals where maize is staple food could contribute significantly to improving the population nutritional status. The objective of this study was to estimate variance and average components related to the maize protein quality based on tryptophan and grain yield analysis, and to select from the early generations progenies which best complement with the tester for both characters. The laboratory analyses were carried out by using colorimetric reactions and the statistical analyses were based on mixed model. The experiment was set out in partially balanced square lattice with 144 treatments, two replications and 12 blocks. The estimations, associated with the assessed treatment performances made it possible to infer that both populations are promising for recurrent selection and suggested good experimental precision. The selective accuracy demonstrated the possibility of obtaining gains by selecting in both characters. For tryptophan content, the specific combining ability presented small magnitude value due the qualitative inheritance and, the additive effects might have been more important. The shrinkage effect in grain yield was more noticeable than the tryptophan content. Over 25% of the progenies contributed positively to the tryptophan content and only 3.65% to grain yield.

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